TY - JOUR T1 - Treatment of recalcitrant vitiligo by autologous non-cultured and trypsinized melanocyte grafting in the west of Iran JO - Anais Brasileiros de Dermatologia T2 - AU - Ghorbani,Iraj AU - Khazaei,Mozafar AU - Kavoussi,Hossein AU - Ebrahimi,Ali AU - Rezaei,Mansour AU - Kavoussi,Reza AU - Mansouri,Kamran SN - 03650596 M3 - 10.1016/j.abd.2021.08.003 DO - 10.1016/j.abd.2021.08.003 UR - http://www.anaisdedermatologia.org.br/en-treatment-recalcitrant-vitiligo-by-autologous-articulo-S0365059622000198 AB - BackgroundVitiligo is a common disease with a high burden, and its recalcitrant type is unresponsive to current medical treatments. Autologous non-cultured and trypsinized melanocyte grafting, which is a simple and experience-based procedure, has been suggested for the treatment of vitiligo. ObjectiveTo assess autologous non-cultured and trypsinised melanocyte grafting in recalcitrant vitiligo. MethodsThis clinical trial was done on 28 patients (20 females and 8 males). After demarcation and preparation of both donor and recipient sites, both sites were shaved by a curette. The materials harvested from the donor site were trypsinized and centrifuged. The resulting suspension was mixed with hyaluronic acid gel and was spread over the shaved recipient area. ResultsTwenty-eight patients with a total of 108 lesions and a mean age of 25.93 ± 7.11 years were included in the present study. Generalized vitiligo (57.1%) was the most common clinical type and the face and neck regions (38%) were the most frequent treated sites. Good to excellent repigmentation was seen in the face and neck, trunk, upper extremity, and genitals in 31 (57.4%), 11 (20.4%), 9 (16.7%) and 3 (5.5%) patients, respectively. Face and neck showed significantly better results (p < 0.05). Study limitationsLow sample size and single-center study. ConclusionAutologous non-cultured and trypsinized melanocyte grafting is a safe method with satisfactory outcomes in recalcitrant vitiligo. Appropriate training of physicians and proper use of specialists’ experiences can be effective in increasing the improvement rate. ER -